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ATCC
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Santa Cruz Biotechnology
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Image Search Results
Journal: PLoS ONE
Article Title: Sonic Hedgehog-Gli1 Signaling Pathway Regulates the Epithelial Mesenchymal Transition (EMT) by Mediating a New Target Gene, S100A4, in Pancreatic Cancer Cells
doi: 10.1371/journal.pone.0096441
Figure Lengend Snippet: M: DNA Marker; PC: RNA polymerase II antibody for XChIP positive control; IP: Gli1 XChIP; IGG: mouse IgG for XChIP random control; NC: β-actin antibody for XChIP negative control.
Article Snippet: ChIP-grade goat polyclonal Gli1 antibody (0.1 µg/mL) (Santa Cruz Company) was used for immunoprecipitation, the mouse IgG (0.1 µg/mL) (
Techniques: Marker, Positive Control, Negative Control
Journal: Cell Death and Differentiation
Article Title: AIF loss deregulates hematopoiesis and reveals different adaptive metabolic responses in bone marrow cells and thymocytes
doi: 10.1038/s41418-017-0035-x
Figure Lengend Snippet: a Left, ECAR of AIF+/Y and AIF−/Y HSC and progenitors cells measured in response to sequential addition of Glucose, Oligomycin, and 2-deoxyglucose (2-DG). Arrows indicate the time of the addition of each reagent. Right, ECAR of HSC and progenitor cells after Glucose treatment expressed as a histogram (n = 3 independent experiments). b–d Mitochondrial ROS generation (MitoSOX labeling) b, DNA damage (γH2AX levels) c, and cell death (Annexin-V/7AAD co-staining) d were evaluated in AIF+/Y and AIF−/Y LT-HSC, ST-HSC, MPP cells from 7-day-old mice. Data are the fold change recorded in the AIF−/Y cells relative to the basal levels of the AIF+/Y cells (n = 5 mice per group). e Mitochondrial ROS levels measured by flow cytometry in AIF+/Y and AIF−/Y BM cells in the presence or absence of NAC (n = 6 mice per group). f Colony counting of BM AIF+/Y or AIF−/Y cells plated in methylcellulose in the presence or absence of NAC (n = 5 independent experiments). Representative images of the morphology of the different colonies are shown. g Dams were supplied or not with NAC in drinking water and the number of LT-HSC, ST-HSC, and MPP cells from 21-day-old AIF+/Y and AIF−/Y newborn mice was counted (n = 7 mice per group). Statistical significance was calculated by Mann–Whitney (a–e and g or two-way ANOVA f tests. Bars represent mean ± SEM
Article Snippet: Apoptosis (cell death) was assessed by flow cytometry using
Techniques: Labeling, Staining, Flow Cytometry, MANN-WHITNEY